Amino Acid Fermentation: Evaluation Of Scale-Down Capabilities

Optimization of bioprocesses needs accurate monitoring and control while small working volumes are saving media and other resources. Evonik® has established a 2 L process for production of a nutritionally relevant amino acid in E. coli. This application note describes how this process was adapted to the smaller working volume of a DASbox Mini Bioreactor System.

Multiple runs were performed with close monitoring of all relevant process parameters and comprehensive evaluation of data to prove reliable and reproducible results. The scale-down capabilities of the DASbox system were evaluated by comparing the fermentation results to the data collected at 2 L scale.