Application Note

An Assessment Of Bacterial Load In Raw Milk Using GreenLight® Technology

Source: MOCON, Inc.

The GreenLight® system was designed for applications across the food industry with further uses in environmental measurements. The core technology is a novel oxygen-depletion sensor that can detect very small changes in oxygen content making the system ideal for use in enumerating aerobic microbes. Here we describe a small comparative study conducted by Luxcel Biosciences, assessing correlation between the GreenLight system and standard plate count (SPC) for local raw milk. GreenLight demonstrated strong correlation to SPC with improvements in time-to-result, limit of detection and preparation costs over SPC. GreenLight has been AOAC and Microval certified for raw meats and poultry and further certifications in dairy products is ongoing.

GreenLight Model 930 is designed to address the increasing demand for faster, simpler methods of determining bacterial load in food samples. The industry standard for TVC determination (ISO 4833:2003), also known as Standard Plate Count (SPC), is widely used but presents users with some very significant drawbacks. The method is both material and labor intensive, requiring the preparation and analysis of multiple agar plates per sample. More importantly, the method is slow, with 48- 72 hours typically required for a definitive result [1-3] and determinations are inherently subjective.

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