The Thermo Scientific ToxInsight IVT Platform
For scientists wishing to profile samples for toxic effects and action mechanisms, the Thermo Scientific ToxInsight IVT Platform offers a complete solution to significantly enhance in vitro toxicology (IVT) assessment. Designed to simplify your workflow by multiplexing targets in each cell, The ToxInsight IVT Platform can provide a clearer picture of potential toxic liabilities.

Traditional methods of assessing toxicity in vitro measure single targets in homogeneous formats and have a record of low predictive power. The ToxInsight IVT Platform leverages the proven power of automated cellular imaging to measure multiple toxicity indicators in each cell of the exposed population. Combined cellular outputs are automatically compared to untreated populations producing a more sensitive, multi-faceted risk assessment tool for in vitro toxicity testing.

The ToxInsight IVT Platform offers intuitive software, ready-made reagent cartridges and easy-to-interpret data analysis tools. The detailed image cytometry data acquired by multi-channel fluorescent imaging of cells in vitro allows analysis on a cell-by-cell basis, providing immediate phenotypic results that cannot be obtained by traditional biochemical assays.

Capabilities

• Superior sensitivity and specificity via unique assay endpoints including cell morphology and intracellular detail.
• Visual confirmation of results – if desired – by examining cellular images.
• A range of Thermo Scientific ToxInsight Cartridges offer appropriate reagents and analysis protocols for each toxicity target, with no assay development required
• Fully automated data capture and analysis.
• Compatible with samples in SBS-compliant micro-well plates.
• Innovative analytics provide a simple-to-interpret report on toxicity risk of a sample

Advantages of the ToxInsight IVT Platform

• Traditional methods of assessing toxicity measure single targets and have a record of low- predictive power.
• The ToxInsight IVT Platform leverages the proven power of cellular imaging, which combines multiple toxicity targets in single cells to provide better indicators of compound toxicity.
• Deeper insight into the mechanism of toxicity through multiple assay endpoints, measured on a cell-by-cell basis.
• ToxInsight endpoints provide greater sensitivity and specificity compared to traditional methods.
• Multiple assay parameters; offer reduced sample usage and rapid workflow.

Reader & Software

• Complete automation of image acquisition and analysis processes. No 'tuning' required.
• Simple-to-use software fits your workflow and gives results in minutes.
• No image analysis experience required.
• Small, bench top footprint.
• Ultra-stable solid-state illumination source offers maintenance free usage and robust data.
• Compatible with plate handling robots.

Data Analysis & Reporting

• Integrated software provides customizable reports that assist evaluation of the toxicity of compounds.
• Dose response plotting and EC/IC50 computation.
• Drill-down to cell-level measurements or images as desired.
• Export of data to popular third party software.

The Thermo Scientific ToxInsight Endocrine Profiler Panel
The Thermo Scientific ToxInsight Endocrine Profiler Panel (EPP) offers a complete solution for consumer products companies needing to comply with regulatory Reproductive Toxicity testing requirements. Traditional methods of assessing endocrine disruption in vitro measure single targets in homogeneous formats and have a record of low-predictive power. The ToxInsight Endocrine Profiler Panel leverages our proven technologies for automated cellular imaging and analysis for assessing the risk of human exposure to endocrine-active chemicals in the environment and measures estrogen and androgen receptor activity in human cell lines.

Each cartridge includes all the cell lines, reagents, assay protocols, growth media, plastic ware, analysis, and data-reporting capabilities to profile compounds for endocrine activity with high-sensitivity and specificity. The ToxInsight EPP cartridges, coupled to the ToxInsight Consumer and Health Products Safety (CHPS) Platform, offer a high-throughput, quantitative pre-screen for primary toxicology assessment, as well as a selection of chemicals for follow-up animal studies, in a simple mix-and-read format that is easily standardized. This automated, bench-top platform is easy-to-use and provides physiologically relevant toxicity assays in vitro, reducing the reliance on late-stage histopathology or animal studies. The ToxInsight CHPS Platform not only profiles compounds for their toxicity risk, but also determines the mechanism of action of any toxicity detected.