Presentation
Presentation: Method Transfer Between HPLC And UHPLC: Issues And Solutions
A natural requirement for UHPLC instruments is to have minimized gradient delay and peak dispersion. Systems are typically designed for the operation of small bore columns (2.1 mm-3.0 mm internal diameter) packed with small particles (1.8 µm) to achieve highest separation efficiencies. With regard to solvent delivery, these systems usually differ significantly from standard HPLCsystems. The most obvious aspect is the dwell volume of such UHPLC systems, which is purposely minimized to allow fast gradient separations.
The content you requested is only available to registered users of Food Online. By registering now, you'll get exclusive access to this piece of content and thousands of addtional articles and product reviews across our entire network of sites.
